Description

The Beckman Center for Cryo-EM is seeking a Sr. Research Specialist II who is an expert electron microscopist to support the center's operations in cryo-electron tomography (cryo-ET), focusing on lamella preparation and cryo-correlation with focused-ion beam scanning electron microscopy (FIB-SEM) and cryo-correlative light electron microscopy (Cryo-CLEM). The Beckman Center houses state-of-the-art instrumentation and support infrastructure including the Titan Krios equipped with a Falcon 4i direct electron detector and Selectris energy filter, a Glacios equipped with a Falcon 4i direct electron detector, and an Aquilos II cryo-CLEM/FIB-SEM with iFLM. The Sr. Research Specialist II uses specialized scientific knowledge and experience to make decisions, trains/guides other laboratory personnel, and provides simple to complex troubleshooting.

Specific Duties & Responsibilities

• Training and assisting users with grid preparation, FIB-SEM, and cryo-CLEM for 3D cryo-ET analysis (primarily utilizing the Aquilos II and associated equipment).
• Day-to-day instrument operation, including FIB milling of cells and tissue, image acquisition, and microscope maintenance.
• Providing user support for image processing and tomographic analysis.
• Maintain high-throughput software pipelines.
• Develop, design and manage specialized projects and/or instrumentation, and coordinates own objectives.
• Utilize specialized knowledge to set up and run sophisticated instrumentation and interpret results.
• Act as consultant to refine or adapt methodologies to fit research objectives.
• Collaborate in development of new protocols and uses of equipment.
• Advise investigators on experimental design and research methods.
• Co-author/author publications.
• Contribute original thoughts, hypotheses, and analysis to write reports, abstracts and manuscripts.
• Provide significant input to research design and data collection and interpretation based on advanced knowledge/ understanding of activity.
• Independently perform research assays specific to the research laboratory interests.
• Collaborate with other lab personnel, managers, and faculty investigators.
• Independently develop protocol and criteria.
• Make decisions regarding research projects that may require assessment, revision of objectives, or altering scientific direction.
• Consider resources and laboratory priorities when making decision.
• Other duties as assigned.

Additional Skills, Knowledge and Abilities

• Significant hands-on FIB-SEM experience, experience in cryo-electron microscopy (cryo-ET / cryo-EM), image processing, and structure modeling or comparable experience and expertise with the relevant techniques.

• The successful candidate must have strong interpersonal communication skills and be able to effectively work with faculty, staff, postdoctoral fellows, and students from a variety of scientific and cultural backgrounds.

Technical Skills & Expected Level of Proficiency

• Data Management and Analysis - Developing
• Experimental Design and Execution - Developing
• Laboratory Troubleshooting and Technical Problem Solving - Developing
• Project Management - Developing
• Protocol Development and Optimization - Developing
• Scientific Interpretation and Consultation - Developing
• Scientific Writing - Developing

The core technical skills listed are most essential; additional technical skills may be required based on specific division or department needs.

Minimum Qualifications

• Bachelor's Degree in Biology, Chemistry or related field.
• Five years of experience in a comparable laboratory performing relevant techniques.
• Additional education may substitute for required experience and additional related experience may substitute for required education permitted by the JHU equivalency formula beyond a high school diploma/graduation equivalent, to the extent

Preferred Qualifications

• Ph.D. Degree in a relevant field
• Master's Degree with related graduate research.

Classified Title: Sr. Research Specialist II
Role/Level/Range: ACRP/04/ME
Starting Salary Range: $64,600 - $113,300 Annually ($89,301 targeted; Commensurate w/exp.)
Employee group: Full Time
Schedule: Monday-Friday, 8:30am-5pm
FLSA Status: Exempt
Location: School of Medicine Campus
Department name: SOM Biophy Biophys and Biophys Chemistry
Personnel area: School of Medicine